Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 0.2M NaCl, 50% glycerol, 5mM DTT. . NHLH2 is a member of the basic helix-loop-helix (bHLH) transcription factor family and is expressed in developing and adult neuroendocrine tissues such as the pituitary and hypothalamus. Recombinant human NHLH2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.