Recombinant Human Fizzy-related protein homolog (FZR1)
Description
Purity > 90% as determined by SDS-PAGE. MW: 70.8kDa. Full Length of Isoform 2: 1-493aa. N-terminal 6xHis-SUMO-tagged. Homo sapiens (Human). Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacent of CDC20, and activates the APC/C during anaphase and telophase. The APC/C rains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate praturely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.