Purity: > 95% by SDS - PAGE Format: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol . MAT1A catalyzes the formation of S-adenosyltransferase (AdoMet) for methionine catabolism in the liver. MAT1A expression also correlates with a differentiated phenotype, whereas liver cells expressing MAT2A present a dedifferentiated phenotype and lowered AdoMet synthesis. Likewise, NFkappaB and TNFalpha cause a switch from MAT1A to MAT2A expression in human hepatocellular carcinoma (HCC), which facilitates cancer cell growth. Recombinant human MAT1A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.