Purity: > 95% by SDS - PAGE Format: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol. PPCS, also known as phosphopantothenate--cysteine ligase, catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. Recombinant human PPCS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.