Purity: > 90% by SDS - PAGE Format: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl , 0.1mM PMSF . Hexokinase2 is one of four highly homologous hexokinase isoforms in mammalian cells. Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria. Recombinant human Hexokinase2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.