Purity: > 95% by SDS - PAGE Format: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 30% glycerol, 1mM DTT, 200mM NaCl . uBE2F, also Known as ubiquitin-conjugating enzyme E2 F, belongs to the ubiquitin-conjugating enzyme family and uBE2F subfamily. ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. uBE2F accepts the ubiquitin-like protein NEDD8 from the uBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human uBE2F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.