Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.. uBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. This protein in vitro catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination. uBE2W is widely expressed, especially at highest levels in testis. Recombinant human uBE2W protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.