Purity: > 90% by SDS - PAGE Format: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT. uFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1. uFM1 is activated by a novel E1-like enzyme, uBA5, by forming a high-energy thioester bond. Activated uFM1 is then transferred to its cognate E2-like enzyme, uFC1, in a similar thioester linkage. Recombinant human uFC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.