Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 0.1M NaCl, 1mM EDTA. ADP-ribosylation factor-like protein 5B, also known as ARL5B, belongs to a family of proteins that are structurally similar to ADP-ribosylation factors. ARLs and ARFs are part of the RAS superfamily of regulatory GTPases. ARL5B is most closely related to ARL5, with which it shares 80% sequence identity. Human ARL5B shares 100% identity with mouse ARL8 and 71% identity with the Drosophila homolog. Two isoforms of ARL8 exist as a result of alternative splicing events. Recombinant human ARL5B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.