Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl. GMP synthase, also known as GMPS, is involved in purine biosynthesis. Existing as a homodimer, GMPS catalyzes the last step in the GMP synthesis pathway, namely the ATP-dependent amination of XMP to GMP. GMPS contains one GMP-binding domain and one glutamine amidotransferase type-1 domain through which it conveys its catalytic activity. Chromosomal translocations involving the gene encoding GMPS are associated with acute myeloid leukemias, suggesting a possible role for GMPS in carcinogenesis. Recombinant human GMPS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.