The competitive Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine) ELISA kit uses the microtiter plate format. Adrenaline, noradrenaline and dopamine, respectively, are bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free Antigens and free Antigens-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample. Sample Types: urine, plasma. Range: Adrenaline: 0.5 - 250 ng/ml, Noradrenaline: 1.5 - 500 ng/ml, Dopamine: 1.5 - 2560 ng/ml. Sensitivity: URINE: Adrenaline: 0.16 ng/ml, Noradrenaline: 0.85 ng/ml, Dopamine 0.46 ng/ml, PLASMA: Adrenaline: 0.8 pg/ml, Noradrenaline: 43 pg/ml, Dopamine 23 pg/ml. Time: Overnight ? The Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine) ELISA assay kit provides materials for the quantitative measurement of adrenaline, noradrenaline and dopamine in plasma and urine. Noradrenaline, adrenaline and dopamine are extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline, N-acyladrenaline and N-acyl-dopamine and then converted enzymatically into N-acylnormeta nephrine, N-acylmetanephrine and N-acyl-3-methoxytyramine.