Flow Cytometry, Immunohistochemistry, Immunoprecipitation. Anti-CD45R (B220) is useful for Flow Cytometry, Immunoprecipitation, and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
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Description
Phycoerythrin conjugated CD45R (B220) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45R. Cross reactivity is observed with human and Feline. Cross-reactivity with CD45R from other sources has not been tested. Anti-CD45R is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.
Immunogen
Anti-CD45R (B220) Antibody (Monoclonal) was produced by repeated immunizations with Abelson murine leukemia virus-induced pre-B tumor cells.
Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.Kishihara K, et al. 1993. Cell 74:143.