P-Selectin, an adhesion molecule, belongs to the selectin/LECAM family. P-Selectin consists of one Lectin domain and several EGF-like repeats, which are followed by a transmembrane region and a short cytoplasmic tail of 35 amino acids. P-Selectin is expressed in endothelial cells and megakaryocytes. In unstimulated cells, P-Selectin is synthesized and stored in the alpha-granules of platelets and the Weibel-Palade bodies of endothelial cells. Upon activation or exposure of stimuli, such as thrombin and histamine, P-Selectin is rapidly translocated from storage granule to the cell membrane within minutes and then is recycled back via endocytosis. Inflammatory stimuli, including TNF-α and endotoxins, can induce P-Selectin expression at the transcriptional and protein levels in mouse endothelial cells, but not in HUVEC. IL-4 and oncostatin M can induce P-Selectin expression in HUVEC cells. Surface P-Selectin binds to its ligand, PSGL-1, which is a glycoprotein that is expressed in neutrophils, monocytes, and dendritic cells. Its interaction initiates cell contact and selectin-mediated leukocyte rolling on the endothelial cells’ surfaces. In addition, some evidence has been shown that P-Selectin contributes to cancer metastasis. The interaction between tumor cells and platelets via P-Selectin facilitates adhesion of tumor cells in the vasculature.
Size
10 μg
Concentration
n/a
Applications
BA
Other Names
CD62 antigen-like family member P (CD62P), Granule membrane protein 140 (GMP-140), Leukocyte-endothelial cell adhesion molecule 3 (LECAM3), Platelet activation dependent granule-external membrane protein (PADGEM)