The Annexin V-PE Apoptosis Detection Kit Plus is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need of fixation. The Annexin V-PE Apoptosis Detection Kit Plus includes annexin V-PE, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeable to live cells and apoptotic cells, but stains