Purity >98%. 4-Methylumbelliferyl-Alpha-L-iduronide (free acid) is a fluorogenic substrate for Alpha-L-iduronidase, an enzyme found in cell lysosomes that is involved in the degradation of glycosaminoglycans such as dermatan sulfate and heparin sulfate. 4-Methylumbelliferyl-Alpha-L-iduronide is cleaved by Alpha-L-iduronidase to release the fluorescent moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases. This substrate is used in assays that measure the activity of Alpha-L-iduronidase, which is commonly deficient in a type of lysosomal storage disease called mucopolysaccharidosis.