Quantitative colorimetric assay. This assay has high sensitivity and excellent specificity for detection of Mini Samples Tumor Necrosis Factor Alpha (TNFa). No significant cross-reactivity or interference between Mini Samples Tumor Necrosis Factor Alpha (TNFa) and analogues was observed. Sample types: cell culture supernatants, cell lysates, plasma, serum, tissue homogenates. Range: 15.6 - 1000 pg/ml. Precision: Intra-Assay: CV<10% / Inter-Assay: CV<12%. Time: 3 hours