Purity >95% by SDS-PAGE, MW: 17 kDa (SDS-PAGE). Sharing 79% sequence identity with human Tumor Necrosis Factor-alpha (hTNF-a), mouse Tumor Necrosis Factor-alpha (mTNF-a) is a cytokine mainly expressed by immune cells. A type II transmembrane protein, TNF-a is further proteolytically processed to a soluble form. The trimeric active TNF-a then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2. Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-a) produced in P.pastoris is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.