The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of C4a in Rat serum, plasma and other biological fluids This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C4a. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat C4a and Avidin-Horseradish Peroxidase (HRP) conj