This ELISA kit applies to the invitro quantitative determination of RatC5aconcentrations in serum, plasma and other biological fluids.This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C5a. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C5aand Avidin-Horseradish Peroxidase (HRP) conjugate