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Description
WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al. ,2002 [PubMed 11756452]). [supplied by OMIM,Mar 2008]Non-catalytic component of the 20S PRMT5-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4),methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Subunit structure: Component of the methylosome,a 20S complex containing at least PRMT5,CLNS1A and WDR77. Directly interacts with PRMT5,as well as with several Sm proteins,including SNRPB and SNRPD2 and,more weakly,SNRPD3 and SNRPE. Forms a compact hetero-octamer with PRMT5,decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/HIST2H2AC,but not with histones H2B,H3 nor H4. Interacts with CTDP1 and LSM11. Interacts with APEX1,AR and NKX3-1. Subcellular location: Nucleus. Cytoplasm. Sequence similarities: Contains 7 WD repeats.
Immunogen
Purified recombinant human WDR77 protein fragments expressed in E.coli.