Western Blotting (WB),Immunohistochemistry (IHC),Immunocytochemistry (ICC)
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Description
PRMT6 is a protein arginine N-methyltransferase, and catalyzes the sequential transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation is a prevalent post-translational modification in eukaryotic cells that has been implicated in signal transduction, the metabolism of nascent pre-RNA, and the transcriptional activation processes. IPRMT6 is functionally distinct from two previously characterized type I enzymes, PRMT1 and PRMT4. In addition, PRMT6 displays automethylation activity, it is the first PRMT to do so. PRMT6 has been shown to act as a restriction factor for HIV replication. Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC). Subunit structure: Interacts with (and methylates) HIV-1 Tat, Rev and Nucleocapsid protein p7 (NC). Subcellular location: NucleusSequence similarities: Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily.
Immunogen
Purified recombinant human PRMT6 protein fragments expressed in E.coli.
Other Names
CG9927|DART6|Dmel\\CG9927|PRMT6|HRMT1L6|ARABIDOPSIS THALIANA PROTEIN ARGININE METHYLTRANSFERASE 6|ATPRMT6|protein arginine methyltransferase 6|DKFZp459B1431|Hrmt1l6|hrmt1l6|hrmt6|im:6908706|AW124876|BB233495