This CLIA kit uses Competitive-CLiA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse NA/NE. During the reaction, Mouse NA/NE in the sample or standard competes with a fixed amount of Mouse NA/NE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse NA/NE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and inc