This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse NANOG monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in pe
[homeobox protein NANOG isoform 2; Homeobox protein NANOG; homeobox protein NANOG; ES cell-associated protein 4; homeobox transcription factor Nanog; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Nanog homeobox; ES cell-associated protein 4; Early embryo specific expression NK-type homeobox protein; Homeobox transcription factor Nanog]