MW: 175.5 kDa. This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KDM5B(KDM5B) (NM_006618), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix and 1mM Na3VO4).