The single-analyte FluoroSpot assay provides an ELISpot alternative with fluorescent detection instead of enzymatic. The assay enables enumeration of cells secreting human Interleukin-12 (IL-12)/Interleukin 23 (IL-23) p40. FluoroSpotBASIC (550) contains monoclonal capture and detection antibodies, a secondary fluorophore-conjugated detection reagent (SA-550) and a Fluorescence enhancer. Cross-react with IL-12/-23 (p40) from non-human primates.