Human Bronchial Smooth Muscle Cells from AcceGen Biotech Research Laboratories are isolated from human bronchi and bronchioles. HBSMC are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBSMC are characterized by immunofluorescent method with antibodies to smooth muscle actin and desmin. HBSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBSMC are guaranteed to further expand for 15 population doublings at the condition provided by AcceGen Biotech Research Laboratories.