The Human Bone Metabolism Panel 1 Mix and Match Capture Beads offer flexible customization within the panel (13-plex) with the following items listed below: 740660 LEGENDplex? Human OPG Capture Bead A4, 13X 740661 LEGENDplex? Human OPN Capture Bead A5, 13X 740662 LEGENDplex? Human PDGF-BB Capture Bead A6, 13X 740663 LEGENDplex? Human ALPL Capture Bead A7, 13X 740664 LEGENDplex? Human ACP5 Capture Bead A8, 13X 740665 LEGENDplex? Human Leptin Capture Bead A10, 13X 740666 LEGENDplex? Human RANKL (TRANCE) Capture Bead B2, 13X 740053 LEGENDplex? Human TNF-α Capture Bead B3, 13X 740667 LEGENDplex? Human IL-6 Capture Bead B4, 13X 740668 LEGENDplex? Human PTH Capture Bead B5, 13X 740669 LEGENDplex? Human IL-1β Capture Bead B6, 13X 740670 LEGENDplex? Human BMP-2 Capture Bead B7, 13X 740671 LEGENDplex? Human DKK-1 Capture Bead B9, 13X Selected items will be used together with 740658 LEGENDplex? Human Bone Metabolism Panel 1 Standard 740659 LEGENDplex? Human Bone Metabolism Panel 1 Detection Antibodies 740623 LEGENDplex? Buffer Set J (Set Up Beads, Buffers, Plate Sealers, SA-PE, Matrix, and Data Analysis Software Dongle) 740377 or 740379 Filter Plate or V-bottom Plate The LEGENDplexTM Human Bone Metabolism Panel (13-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. It allows for simultaneous quantification of 13 key targets involved in bone metabolism including OPG, OPN, PDGF-BB, ALPL, ACP5, Leptin, RANKL, TNF-α, IL-6, PTH, IL-1β, BMP-2, and DKK-1. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum, and plasma samples.