ELISA, Western Blot. This antiserum against Human GM-CSF has been tested for use in neutralizations, ELISA and immunoblotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method.
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Description
This antiserum has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antiserum will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of human GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with full length recombinant human GM-CSF.