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Description
Goat IgG Against Mouse IgM (FITC). The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule. In immunoelectro- phoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma and serum, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No reaction is obtained with purified IgA, IgG and IgG/Fab fragments. Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of foreign protein or immune complexes in the antiserum.
Immunogen
Purified homogenous IgM isolated from pooled mouse serum. Immunization with intact (19S) and split (7S) IgM. Freunds complete adjuvant is used in the first step of the immunization procedure.