The determination of rat IgG is carried out as direct sandwich ELISA. An antibody specific for rat IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG con cen tration. Sample Types: serum, biological fluids. Range: 15.6 - 50 ng/ml. Sensitivity: 15.6 ng/ml (sample dilution 1:10). Time: 2.5 hours ? The Eagle Biosciences Rat IgG ELISA Assay Kit is designed for the quantitative determination of rat IgG in complex samples (serum or other biological samples). The Rat IgG ELISA Assay Kit is for research use only and should not be used in diagnostic procedures.