Differential medium for identification of enteric bacteria based on fermentation of dextrose, lactose and H2S production. This medium permits differentiation of Gram-negative bacilli by their ability to ferment Dextrose or Lactose, and produce hydrogen sulfide. Enzymatic Digest of Casein and Enzymatic Digest of Animal Tissue provide nitrogen, carbon, and vitamins required for organism growth. Ferric Ammonium Citrate and Sodium Thiosulfate are indicators of hydrogen sulfide production. Phenol Red is the pH indicator. Sodium Chloride maintains the osmotic balance of the medium. Agar is the solidifying agent. Formulation (g/L): Enzymatic Digest of Casein 10 Enzymatic Digest of Animal Tissue 10 Lactose 10 Dextrose 1 Ferric Ammonium Citrate 0.5 Sodium Chloride 5 Sodium Thiosulfate 0.5 Phenol Red 0.025 Agar 15 Total: 52g/L Directions per Liter: 1. Suspend 52g of the medium in one liter of ddH2O. 2. Heat with frequent agitation and boil for one minute to completely dissolve the medium. 3. Dis