Purity >90%. Cal Green(TM) 1 AM is a cell-permeant acetyloxy-methyl ester compound that is converted to the fluorescent calcium indicator Cal Green(TM) 1 by intracellular esterases. This derivative is characterized by high quantum yield and low phototoxicity. Its peak excitation and emission wavelengths (506 and 531 nm, respectively) are comparable to standard fluorescein dyes, making Cal Green(TM) 1 AM appropriate for fluorescent microscopy and flow cytometry. Cal Green(TM) 1 is ~5-fold brighter than fluo-3 at saturating calcium levels.
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Other Names
N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[2-[2-[5-[[[3',6'-bis (acetyloxy)-2',7'-dichloro-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]amino]-2-[bis [2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]-glycine, (acetyloxy)methyl , Calcium Green-1 AM