Sequence: 5'-G T A T C C (N)6^-3' 3'-C A T A G G (N)5^-5' Source: Bacillus firmus Auk Concentration: 5u/ul Unit Definition: One unit is defined as the amount of BfuI required to digest 1ug of lambda DNA dcm in 1 hour at 37°C in 50ul of reaction buffer. Supplied With: R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA (pH 7.9 at 37°C). R1625-20: Restriction Enzyme Buffer for Bful, 10X: Supplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25°C), 300mMNaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Storage and Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Thermal Inactivation: Bful is inactivated by incubation at 80°C for 20min.