ELISA, IF Microscopy, Immunoprecipitation, Western Blot. Anti-IDO2 antibody has been tested for use in ELISA, IF, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 45.4 kDa in size corresponding to IDO2 protein by western blotting in the appropriate cell lysate or extract. IHC testing has been negative.
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Description
Anti-IDO2 antibody is directed against IDO2 protein. The rabbit polyclonal antibody was affinity purified against the antigen. Prior to affinity purification the antibody was pre-cleared of any cross reactive antibody to IDO1 (human and mouse). A BLAST analysis was used to suggest cross-reactivity with IDO2 protein from human and mouse 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Immunogen
IDO2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a mixture of human and mouse full length protein corresponding to IDO2.
Other Names
rabbit anti-IDO2 antibody, IDO 2, IDO-2, Indoleamine 2,3-dioxygenase 2, Indoleamine 2,3-dioxygenase-like protein 1, INDOL1, Indoleamine-pyrrole 2,3-dioxygenase-like protein 1
Metz, R., DuHadaway, J., Rust, S., Muller, A. and Prendergast, G. (2010) The Antitumor Properties of Zinc Protoporphyrin IX, a Heme Oxygenase-1 Inhibitor, Is Dependent in Part on Functional IDO1. MolCancer Ther. Jun; 9(6):1864-71.