Acetylcholinesterase (AChE) is one of the most crucial enzymes associated with nerve response and function. AChE is a serine protease that hydrolyzes the neurotransmitter acetylcholine. Irreversible inhibition of AChE may lead to muscular paralysis, convulsions, bronchial constriction, and death by asphyxiation. Sample Type: Serum, plasma or blood. Animal tissues: liver, heart, kidney, etc. Cell culture: Adherent or suspension cells. Intended Use: For use in measurement of AChE activity in various tissues/cells and for screening of AChE inhibitors. Sensitivity: This assay kit can detect acetylcholinesterase activity as low as 0.5mU/ml in a variety of samples. Test Principle: This Acetylcholinesterase Activity BioAssay? Kit provides a simple and sensitive method for continuous monitoring of enzymatic activity using colorimetry (OD 570nm). In this assay, AChE converts acetylcholine substrate to choline, which is then oxidized by choline oxidase (CO) to produce an intermediate. The