The Cell Proliferation Assay Kit (Fluorometric) provides an easy and quantitative method to measure cell proliferation and cytotoxicity. The assay is based on a nuclear dye that specifically binds to nucleic acid in the cell and generates green fluorescence. The generated fluorescent intensity is directly proportional to the cell number, which can be quantified by measuring fluorescence (Ex/Em = 480/538 nm). This assay kit provides a just add-and-read, non-radioactive, and high-throughput method for cell proliferation and apoptosis. The assay is rapid and more sensitive than MTT, XTT, or MTS-based assays and can detect a wide linear range of 25-60,000 cells. Sample types: adherent cells, suspension cells
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Applications
Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents, and other pharmaceuticals. Assessment of physiological mediators and antibodies that inhibit cell growth