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Description
Rabbit IgG polyclonal antibody for NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1(NDUFA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat. No cross reactivity with other proteins. NDUFA1(NADH-UBIQUINONE OXIDOREDUCTASE 1 ALPHA SUBCOMPLEX, 1), also called MWFE, B. TAURUS, HOMOLOG OF, encodes a subunit of mitochondrial NADH: ubiquinone oxidoreductase, also known as mitochondrial complex I. The NDUFA1 gene is mapped to chromosome Xq24. The deduced polypeptide sequence of NDUFA1 was found to have an N-terminal hydrophobic domain, likely to be a transmembrane domain, and a C-terminal hydrophilic domain. And the NDUFA1 gene contains 3 exons and spans about 5.0 kb of genomic DNA. Complementation with hamster Ndufa1 cDNA restored the rotenone-sensitive complex I activity of these mutant cells to approximately 100% of the parent cell activity. The findings established that the MWFE polypeptide is absolutely essential for an active complex I in mammals. The NDUFA1 peptide is one of about 31 components of the hydrophobic protein(HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFA1(54-70aa ISGVDRYYVSKGLENID), different from the related rat and mouse sequences by one amino acid.