Human TNF Alpha ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for TNF Alpha has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli,V77-L233) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNF Alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin- Peroxidase Complex was added and