Purified (~62%). Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), GenBank Accession No. NM_004333, a.a.417-end with Val-to-Glu mutation on a.a.600* and N-terminal GST tag, MW=69kD, expressed in Sf9 insect cells via a baculovirus expression system.*This mutation is often cited as V599E. Source: Recombinant protein corresponding to active human BRAF at N-terminal, fused to GST tag expressed in Sf9 insect cells. Molecular Weight: ~69kD Specific Activity: 150 pmol/min/ug Assay Conditions: Assay was performed in a kinase buffer containing 0.1mg/ml inactive MEK1 substrate and 10uM ATP. Reaction was done at 30°C for 30 minutes. ATP transferred was calculated using Kinase-Glo? (Promega). Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.