Purity: > 95% by SDS - PAGE Format: In 50 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT, 10% glycerol. Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E.coli and purified by conventional chromatography techniques.