G6PD, 1-515aa, Human, His tag, Baculovirus (Bioactivity Validated)
Description
Purity: > 95% by SDS - PAGE Format: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1mM PMSF, 2mM EDTA, 2mM DTT, 200mM NaCl. Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant human G6PD protein, fused to His-tag at N-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.