5'-G T C T C (N)1^-3' 3'-C A G A G (N)5^-5' Source: Acinetobacter lwoffi RFL26 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of Alw26I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Incubation Temperature: 37°C Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Restriction Enzyme Buffer A, 10X: R1625: Supplied as a liquid in 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37°C. Supplied to simplify buffer selection for double digests.