Endothelial Progenitor Outgrowth Cells, Mouse, Freezing Medium
Description
Description: Mouse EPOC freezing medium was optimized for cryopreservation of our Mouse endothelium progenitor Outgrowth cells (mEPOC). This product contains fetal bovine serum. Each lot is tested for sterility. Procedure for Freezing mEPOC: 1. Freeze mouse EPOC cells when they reach > 95% confluence. 2. Warm Dulbecco's PBS (without Ca2+ bath. Thaw EPC freezing medium at room temperature. and Mg2+) and 0.05% trypspin/EDTA in a 37°C water. 3. Remove EPOC growth medium, rinse mEPOC with Dulbecco's PBS, and incubate the cells with trypsin/EDTA solution until approximately 90% of the cells begin to detach. Monitor the cells under a microscope to avoid over-trypsinization. 4. Add fetal bovine serum equal to 1/10 volume of the trypsin/EDTA solution to neutralize trypsin. Gently shake the culture vessel to mix. 5. Transfer the cell suspension into a 15ml conical tube. Take a small aliquot of the suspension to count cells. Centrifuge the cell suspension 250 x g for 5 minutes at room temperatur