ERK1 pT202-pY204 is a recombinant protein containing a polyhistidine tag expressed in Sf9. ERK1 was phosphorylated in vitro using the activated MEK. Analysis by SDS-PAGE resulted in a pattern consistent with purified ERK1 and was estimated to be greater than 90% pure.
Size
10 μg
Concentration
n/a
Applications
Western Blot. ERK1 pT202-pY204 is an activated recombinant protein suitable as a control for polyclonal or monoclonal anti-ERK in immunological assays. ERK1 can be activated by in vitro phosphorylation by MEK proteins. For western blot use at 50 ng or less. For other assays concentration is user optimized.