Histone acetylases (HAT's) and Histone deacetylases (HDAC's) are associated with regulation of gene expression. In general, increased levels of histone acetylation are associated with increased transcriptional activity, whereas decreased levels of acetylation are associated with repression of gene expression. HDAC's are localized in both the cytosol and nucleus and some shuttle between the nucleus and cytosol. Increased HDAC expression has been observed in various cancers. BioVision's InSitu HDAC Activity Fluorometric Assay Kit provides a direct, fast, fluorescence-based method to measure the InSitu HDAC activity. The procedure requires two easy steps, all performed in the same cell culture plate. First, the cell permeable HDAC Substrate, w