In situ hybridization (ISH) is a powerful method for gene expression analysis. This In situ Hybridization Kit (ISH), BioAssay? provides the reagents, including hybridization buffer, NBT/BCIP color development solution and washing buffer solutions, for pre-hybridization, hybridization, and post hybridization treatments. This ISH kit provides researchers a method doing in situ hybridization with non-radioactive probes. Compared with radioactive methods, the sensitivity of this kit is the same while the procedure is simplified. This kit is suitable for digoxigenin labled probes combined with an alkaline phosphatase color development system. Using this kit, all disadvantages of working with radioactive materials can be avoided. Features: ? microRNA detection ? Non-isotopic, colorimetric detection of gene expression ? RNase-free, ISH-optimized reagents ? Can be used with either oligonucleotide or ribonucleotide probes ? Tested by Digoxigenin labeled oligonucle