ELISA, Immunohistochemistry, Western Blot. This antibody reacts with human, rat and mouse CAND1 by western blot and immunoprecipita-tion. The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been tested. A 136.4 kDa band corresponding to human CAND1 is detected. Most cell lines expressing CAND1 can be used as a positive control. Researchers should determine optimal titers for other applications.
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Description
This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human, rat and mouse CAND1/TIP120A. Cross reactivity does occur with human, rat and mouse CAND2/TIP120B. Cross reactivity with CAND1 from other sources is not known.
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 11-24 of Human CAND1/TIP120A (N-terminal) coupled to KLH.
Other Names
rabbit anti-CAND1 antibody, Cullin associated NEDD8 dissociated protein 1 antibody, p120 CAND1 antibody, TATA binding protein interacting protein, 20A antibody, TBP interacting protein antibody, TBP interacting protein of 120 kDa A antibody, TIP120 antibody
Feng S, Shen Y, Sullivan J, Rubio C, Xiong Y (2004) The Arabidopsis thaliana CAND1, an unmodified Cul1 interacting protein, is involved in multiple development pathways controlled by ubiquitin/proteasome-mediated protein degradation. Plant Cell. 2004 Jul; 16(7): 1870-1882. Cheng Y, Dai X, Zhao Y. (2004) AtCAND1, a HEAT-repeat protein that participates in auxin signaling in Arabidopsis. Plant Physiol. 135(2):1020-6. Liu J, Furukawa M, Matsumoto T, Xiong Y.(2002) NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol Cell. 10(6):1511-8.