The Eagle Biosciences Human Leptin ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Leptin α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Leptin present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Leptin is added to the wells and binds to the combination of capture antibody- Leptin in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of Leptin present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven Leptin standard dilutions and Leptin sample concentration determined. Sample Types: serum, plasma, cell culture. Range: 31.25 - 1000 pg/ml. Sensitivity: 15 pg/ml. Time: 3.5 hours ? The Eagle Biosciences Human Leptin ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Leptin concentrations in cell culture supernates, serum, and plasma. The Eagle Biosciences Human Leptin ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.