This assay has high sensitivity and excellent specificity for detection of Netrin 4. No significant cross-reactivity or interference between Netrin 4 and analogues was observed. Sample types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Range: 7.8-500 pg/mL. Sensitivity: 3.1 pg/mL. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Netrin 4 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Netrin 4 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV<10%;Inter-Assay: CV<12%. Time: 3 hours