The IL-35 (Interleukin 35) BioAssay? ELISA Kit is a quantitative sandwich assay for the detection of IL-35 in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples. Range: 15.625-1000pg/ml Sensitivity: <9.375pg/ml Precision: Intra-assay CV: <8% Inter-assay CV: <10% Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-35. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to IL-35. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL-35, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measure